Due to that receiving and the discharging of large quantities of warm water (76° F) that enters through underground springs, the environment within the well is very stable. This allows a unique ecosystem with several plants and animals found nowhere else on earth. Leeches, amphipods, water scorpions and turtles live in this closed ecosystem.

However, due to the high concentration of dissolved carbon dioxide, 600 times higher than most natural aquatic environments, there is a relative lack of oxygen, which precluded many aquatic animals from living in these waters, in particular fish.